By G. Inog. University of Wisconsin-Platteville.
Blood agarculture • look for beta-haemolytic colonies that could be group Astreptococcu(S discount 250 mg disulfiram mastercard. Sabouraud agar culture Look for candid albicans 130 Collection transport and examination of Nasopharyngeal aspirates and Nasal swabs Nasopharyngeal Aspirates and perinasal swabs Possible pethogens Grampositive G ram negative Streptococcus pneumonia Haemophylus influenzae Corynebacterium diphtheriae Neisseria meningitidis (carriers) Bordetella pertussis Bordetella parapertussis Klebsiella species Also M purchase disulfiram 250 mg with visa. Anterior Nasal Swabs Possible pathogens • Most anterior nasal swabs are examined to detect carriers of pathogens 131 Gram positive Gram negative S buy generic disulfiram 250mg on line. Using a sterile cotton or alginate wool swab attached to an easily bent pieces of wire, gently pass the swab along the floor of one nostril directing the swabdown wards and backward as far as the Nasopharynx. Using a steile cotton wool swab moistened with sterile peptone water, gently swab the inside surface of the nose. Examine and report the cultures Blood agar and chocolates agar cultures(routine) Look for coloniess that could be H. Collection, Transport and examination of Ear Discharges Possible pathogens Gram positive Gram negative S. A fungal infection of the ear is called otomycosis External Ear infection are more commonly caused by: S. The following organisms may be found as commensals in the external ear: Gram positive Gram negative Viridans streptococci Escherichia coli and other coliforms S. Place it in container of Amies transport medium, breaking off the swab stick to allow the bottle top to be replaced tightly. Label the specimens and send them with its request form to the laboratory Within 6 hours. Additional: Chocolate agar if the patient is a child: Inoculate the specimen on chocolate (heated blood) agar for the isolation of H. Incubate the plate in a carbon dioxide enriched atmosphere at 0 35-37 c for up to 48 hours, examining for growth after overnight incubation. Incubate the plate anerobically for up to 48hours, checking for growth after overnight incubation. Sabouraud agar if a fungal infection is suspected Inoculate the specimen on sabouraud agar, and incubate at room tempreture for up to 6 days. Examine the specimen Microscopically Gram smear - Make an evenly spread of the specimen on a shide. Additional: Potassium hydroxide preparation if a fungal infection is suspected - Mix a small amount of the specimen with a drop of potassium hydroxide, 200g/l (20%W/v) on a slide, and cover with a coverglass. Look for: • Brnaching septate hyphae with small round spores, that could be Aspergillns speies • Pseudohyphae with yeast cells, that could be candida specis (Gram positive) 140 • Branching septate hyphae, that could be a species of der matophyte • Branching aseptate hypae, that could be a species of phycomycete. Inflammation of the the delicate membrane lining the eyelid and covering the eyeball conjunctiva is called conjunctivitis. It causes a severe purulent conjunctivitis that can lead to blindness if not treated. Herpes simplex virus can cause severe inflammation of the cornea (Keratitis) Commensals - That may be found in the eye discharges: Gram positive Gram negative Viridans streptococci Non-pathogenic neisseriae Staphylococci Moraxella speires Collection and transport of eye specimen • Eye specimen should be collected by medical officer or experienced nurses. Using a dry sterile cotton wool swab, collect a specimen of discharge (if an inflant, swab the lower conjunctival surface). Make a smear of the discharge on slide (frosted-ended) for staining by the Gram technique. As soon as possible, deliver the inoculated plates and smear(s) with request form to the laboratory. Culture the specimen Routine: Blood agar and chocolate agar • Inoculate the eye discharge on blood agar and chocolate (heated blood) agar. Loeffler serum slope if Moraxella infection is suspected: • Inoculate the eye discharge on a loeffler serum slope. Microscopically examination Routine: Gram smear Look for:- • Gram negative intracellular diplococci that could be N. If found, a presumptive diagnosis of gonococcal conjunctioitis can be made A cervical swab from the mother should also be cultured for the isolation of N. Depending on the stage of development; If the inclusion body is more mature, it will contain ---- red- mauve stiaing elementary particles. Using a sterile dry cotton wool swab, collect a sample of discharge from the infected tissue. If there is no discharge, use swabmoistened with sterile physiological saline to collect a specimen. If the specimen has been aspirated, transport the needle and syring in a sealed water proof container immediately to the laboratory. Laboratory examination of skin specimens 1) Culture the specimen Blood agar and MacConkey • Inoculate the specimen 0 • Incubate both plate aerobically at 35-37 C overnight.
The size and shape can vary disulfiram 500mg for sale, but it usually is between 3 and 5cm long and is found 10 to 150cm from the ileocecal valve purchase cheap disulfiram line. Meckel’s diverticula contain a mesentery with an independent blood supply from the ileal vessels purchase disulfiram with a visa. Although usually lined by mucosa similar to that seen in the adjacent ileum, approxi- mately 16% to 34% of them can contain heterotopic mucosa, including that of a gastric or duodenal nature. Meckel’s diverticula that contain heterotopic gastric mucosa can produce acid, resulting in ulceration in the adjacent ileal mucosa and subsequent hemorrhage. In fact, hemorrhage is the most common complication associated with Meckel’s diverticulum (31%). The passage of blood per rectum in an otherwise healthy child should raise the suspicion of a Meckel’s diverticulum. The diagnosis can be made utilizing a technetium 99m (Tc-99m) pertechnetate Meckel’s scan that detects the gastric mucosa within the Meckel’s diverticulum and that has been reported to be 90% accurate. Meckel’s diverticulum also can be detected angiographically in most cases, based primarily on the demonstration of a persistant vitellointestinal artery. When a Meckel’s diverticulum causes symptoms or complications, resection is indicated. Other causes can include inﬂammatory bowel disease, neoplasms, ischemic colitis, and a variety of other lesions. Minor bleeding may be related to anal conditions, such as hemorrhoids and anal ﬁssures, or to colonic or rectal lesions, such as neoplasms or mucosal inﬂammation. We focus on the management of patients who present with obvious rather than occult blood loss. Appropriate resuscitation should be initiated as discussed above and as indicated in Algorithms 20. Information provided by the examination, although it may be limited, can give an indication as to the rate of bleeding and to the appearance of the mucosa. The pres- ence of bleeding internal hemorrhoids can be detected by sigmoi- doscopy. However, if an anal source of bleeding is suspected based on the history, then an anoscopy should be performed. These exams still can prove to be unreliable if a large amount of blood remains unevacuated in the rectum. However, it is important to know that this area appears normal in the event that an emergency subtotal or total colectomy may be required. If the patient is stable but has evidence of ongoing bleeding and the sigmoidoscopy is unrevealing, the following diagnostic studies can be considered: emergency colonoscopy, angiography, and radionuclide scanning, with radionuclide scanning being the preferred ﬁrst test. Radionuclide Scanning:Ifbleeding is thought to be ongoing, radio- nuclide scanning may prove to be very useful. Technetium 99m is used because it has a longer half-life and generally less background in the liver and spleen when compared to sulfur colloid. A small aliquot of the patient’s blood is withdrawn, the red blood cells are labeled with Tc-99m, and the blood is returned to the patient. Success in localization is operator dependent and varies widely between institutions, but sensitivities as high as 97% and speciﬁcities of 85% have been reported from multiple centers. Others have had less success, but most centers require this prior to angiography because of the higher sensitivity of the nuclear medicine test compared to angiography: 0. Angiography: Angiographic localization is attempted in those patients with a positive nuclear medicine scan or in whom bleeding is vigorous or has not stopped spontaneously. However, it is helpful only if the rate of bleeding during the procedure is 2mL/min or greater. This technique allows for conﬁrmation of location and therapeutic intervention with either pitressin infusion (0. Both techniques have a greater than 90% success rate, but pitressin infusion has been associated with a 50% in-hospital rebleed rate. Pitressin infu- sion has signiﬁcant cardiac toxicity and requires immobilization in an intensive care unit with a catheter in place, while patients who have had an embolization must be monitored for bowel ischemia, due to a postembolic colon infarction rate that approached 13%. Urgent Colonoscopy: Emergent colonoscopy after rapid bowel cleans- ing has been performed successfully as both a diagnostic and a ther- apeutic technique in select institutions with dedicated teams, but this has not gained widespread acceptance despite excellent results. Corbett workup is not warranted, but colonoscopy should be performed within 24 hours or as soon as feasible. The patients often will not need to have a bowel prep (this refers to the practice of administering oral agents to “clean out” the colon) because blood in the intestine acts as a cathartic agent. In this instance, administration of enemas achieves adequate preparation of the colon. Investigation of Minor Bleeding Passage of small amounts of bright red blood per rectum, either mixed with or on the surface of the stool, can occur. When the bleeding is obvious and bright red, it can be assumed that the blood loss is within or distal to the left colon. If there is obviously an anorectal cause in a young healthy patient, no further workup is necessary. In patients older than 40 years, in patients with a family history of colon cancer, and in patients in whom there is no obvious bleeding source by anoscopy or sigmoidoscopy, an elective colonoscopy fol- lowing a complete bowel prep is warranted.